1. A hybridoma is
1) a hybrid cell obtained by
fusing a β– lymphocyte with a myeloma cell in vitro
2) a hybrid
cell obtained by fusing a β–lymphocyte with a myeloma cell in vivo
3) a hybrid
cell obtained by fusing 2 β–lymphocyte cells in
vitro
4) a hybrid cell obtained by
fusing any 2 body cells in vitro
2. A hybridoma cell
1) produces different types
of antibodies against different types of
antigens
2) produces only specific
antibodies only against a specific antigen
3) produces different types
of antibodies but only one type of
antigen
4) none of the above
3. A cancerous / myeloma
cell in hybridoma helps in
1) Continuous growth of
hybridomas 2) production of antibodies
3) both 1 and 2 4) neither
1 nor 3
4. All the antibodies
produced through hybridoma are
1) polyclonal 2) monoclonal 3) non active 4) over active
5. A type
of β–lymphocyte that produces antibody is
1) plasma cell 2) memory cell 3) adipocyte 4) erythrocyte
6. The unique feature of
monoclonal antibody is that
1) it is specific to a
single antigenic determinant of a single antigen
2) it is non specific
3) it is specific to a few
antigenic determinants
4) restricted growth
7. Monoclonal antibodies are
nowadays used in
1) disease diagnosis
2) detection of specific
type of pathogen
3) very early and accurate
detection of cancer
4) all of these
8. Monoclonal antibodies are
usually produced from
1) myeloma cells 2) hybridoma cells 3) monocytes 4) adipocytes
9. To produce monoclonal
antibodies in large scale, the techniques that can be used are
1) in vivo in the peritoneal
cavity of mice
2) in vitro in large scale
culture vessels
3) both 1 and 2
4) neither 1 nor 2
10. To produce monoclonal
antibodies in large scale
1) stirred bioreactors can
be used
2) air lift fermenters can
be used
3) vessels based on
immobilized cells can be used
4) all of these
11. Which one
of the following statements about Restriction Endonuclease is true
1) all restriction
endonucleases cut DNA at specific sites
2) all restriction
endonucleases cut DNA at random sites
3) all restriction
endonucleases join DNA segments at specific sites
4) all restriction
endonucleases join DNA at random sites
12. Restriction
endonucleases cut DNA at a specific site called
1) ligation site 2) ori
3) recognition sequence 4) replication site
13. Restriction
endonucleases, when present in a host cell act on foreign DNA molecule and
cleave them, but they do not act on host DNA molecule.It happens because
1) Restriction endonuclease
cannot act on host DNA
2) Host DNA is packed into
chromosomes
3) Host DNA is methylated
hence restriction endonucleases can’t act.
4) Restriction endonucleases
become inactive when they reach host DNA
14. The presence of
Restriction endonucleases were postulated in 1960 by
1) Khorana 2) Watson 3) Crick
4) Arber
15. The scientists who won nobel prize for physiology for their discovery of restriction
endonucleases are
1) Jacob and Monad
2)
Smith, Nathans and Arber
2) Watson and Crick 4) Alec Jaffreys and Milstein
20. Restriction
endonucleases are also called
1) Molecular scissors 2) molecular stichers
3) DNA synthesis 5)
polymerases
21. In restriction
endonuclease EcoR1, “E” stands for
1) extraction 2) the first letter of the genus in
which it is present
3) endonuclese 4) endangered
22.EcoR1 cleaves DNA at
1) 5/G AATTC3/ 2) 5/GTT↓AAC3/
3/
CTTAA
G5/ 3/CAA↑TTG5/
3) 5/C↓AATTG3/ 4) 5/GGGCC↓T3/
3/
GTTAA↑C5/
3/CCCGG↑A5/
23. Restriction
endonucleases recognize specific
sequences on DNA called
1) non-coding sequences 2) satellites
3) palindromes with rotational
symmetry 4) tandem repeats
24. Main tools required for
recombinant DNA technology are
1) vector, desired gene
2) vector, desired gene,
mRNA of desired gene, host, restriction enzymes, ligases
3) desired gene, host,vector
4) vector, desired gene,
mRNA of desired gene, host
25. An example for
autonomously replicating mini chromosome is
1) virus 2)
phage 3) plasmid 4) lichen
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