Biotechnology- CET Mock Test Paper-VI and Answers

1. Globular protein when treated with organics solvent get denatured. The main interaction which is affected on treatment with organic solvent is

a) H bonds       b) Covalent bond

c) Ionic bond   d) Hydrophobic interaction.

Answer: d

2. Which of the following organism excrete uric acid?

a) Human   b) Fish

c) Frog        d) Bird

Answer: a

3. Which molecule has property of self replication?

a) Protein    b) Carbohydrate

c) Lipids     d) Nuclei acid

Answer: d

4. Among the following imino acid is

a) Proline          b) Arginine

c) Tryptophan  d) Lysine

Answer: a

5. Exponential growth in bacteria would be expected during

a) lag phase             b) log phase

c) stationary phase  d) deceleration phase

Answer: b

6. The half-life for a zero order reaction is calculated using

a) t1/2= 0.693/k     b) t1/2 = 2.303/k

c) t1/2 = 1/ak         d) t1/2 = a/2k

Answer: d

7. Which cells have high tensile strength?

a) Microbial       b) Plant cells

c) Animal cells  d) none of the above

Answer: b

8. In the bioreactor, plant cell suspensions are viscous at high concentration and behave

a) like Newtonian fluids    b) like Non-Newtonian fluids

c) like Pseudoplastic          d) None of the above

Answer: b

9. Increased viscosity of the culture broth

a) decrease kLa

b) increase kLa

c) increase the mixing and oxygen transfer

d) decrease the mixing and oxygen transfer

Answer: a

10. Bubble column reactor is used for

a) large scale cultivation of plant cells

b) large scale cultivation of microbial cells

c) large scale cultivation of fungi

d) none of the above

Answer: a

11. Airlift bioreactor is a stirred tank reactor with

a). a draught tube is inserted instead of the impeller

b) a low speed agitator is inserted

c) an impeller is inserted in the centre of the reactor

d) high speed agitator is inserted

Answer: a

12. Which of the following is the best definition of generation time?

a) The length of time it takes for lag phase

b) The length of time it takes for a population of cells to double

c) The maximum rate of doubling divided by the initial count.

d) The duration of log phase

Answer: b

13. If you start out with a population density of 200 CFU/ml of a bacterium that divides

every 30 minutes, what will the population density be at the end of two hours, assuming

the cells are in the log phase of growth

a) 800CFU/ml         b) 24CFU/ml

c) 32000CFU/ml    d) 12800CFU/ml

e) 2004CFU/ml

Answer: c

14. A bacterial population increases from 100 to 100,000,000 in 15 hours. What is the

generation time of this culture?

a) 45 minutes

b) 60 minutes

c) 2 hours

d) Not enough information to determine the generation time

Answer: a

15. Use this typical bacterial growth curve to answer the following question.

Which section shows a growth phase where the number of cells dying equals the number of

cells dividing?

a) A         b) B

c) C         d) D

Answer: c

16. Which of the following is used to grow bacterial cultures continuously?

a) Chemostat           b) Coulter Counter

c) Hemostat             d) Petroff-Hausser chamber

Answer: a

17. Which of the following procedures uses a photocell to measure absorbance of a culture to regulate the flow of culture media?

a) Chemostat             b) Hemostat

c) Coulter Counter    d) Turbidostat

Answer: d

18. The total biomass of an organism will be determined by the nutrient present in the lowest concentration relative to the organism's requirements is a statement of

a) Liebig's Law of the minimum

b) Shelford's law of tolerance

c) the second law of thermodynamics quorum sensing

d) Heisenberg's principle of uncertainty

Answer: a

19. An organism is completely dependant on atmospheric O2 for growth.

This organism is A(n)

a) Osmotolerant                     b) Facultative anaerobe

c) Aerotolerant anaerobe      d) Obligate aerobe

Answer: d

20. The ability of Vibrio fischeri to produce bioluminescence chemicals only when a certain

population density has been reached is an example of

a) Liebig's Law of the minimum

b) Shelford's law of tolerance

c) the second law of thermodynamics quorum sensing

d) Quorum sensing

Answer: d

21. Compare the following statement and mark the answer

I. generation time of cells during the exponential phase of growth

II. generation time of cells during the lag phase of growth

a) I is greater than II

b) I is less than II

c) I is exactly or approximately equal to II

d) I may stand in more than one of the above relations to II

Answer: b

22. Reverse transcriptase can be used in

a) cDNA synthesis     b) DNA synthesis

c) RNA synthesis       d) rDNA synthesis.

Answer: a

23. Gene silencing can be achieved by

a) mitochondrial interference     b) DNA interference

c) ribosome interference            d) RNA interference.

Answer: d

24. Transgenic mouse means its genome contains an inserted piece of

a) DNA     b) protein   c) golgi complex  d) carbohydrate.

Answer: a

25. Prokaryotic expression vector can be used to

a) express protein

b) express posttranslationally modified protein

c) express lipid

d) express phospholipids.

Answer: a