1. Prior to the production of recombinant insulin, insulin obtained from cows and pigs were given to patients. Some of the problems faced by this treatment was
1) the insulin was not
active
2) in some humans it induced
antibody production
3) it reduces the weight of patients
4) loss of memory power
2. A plant called Rauolfia serpentina is under the threat of extinction. To save this plant, which technique is highly
useful?
1)genetic
engineering 2) DNA finger printing
3) hybridoma
technology 4) in vitro culture
3. Which group of enzymes
are popularly called “Molecular stichers”
1) Restriction Endonuclease 2)
ligases
3) RNA polymerase 4) DNA polymerase
4. A clone is a group of
organisms produced by
1) asexual method and
genetically similar
2) asexual method and
genetically dissimilar
3) sexual method and
genetically similar
4) sexual method and
genetically dissimilar
5. Match the following:
1) Restriction
endonuclease p. Kary Mullis
2) DNA Finger printing q. Kohler and Milstein
3) Polymerase chain
reaction r. Alec Jaffreys
4) Monoclonal
antibodies s.
Arber
1)1-s, 2-r, 3-p, 4-q 2) 1-s, 2-r, 3-q, 4-p
3)1-q, 2-r, 3-p, 4-s 4) 1-s, 2-p, 3-q, 4-r
6. Some of the steps
involved in Gene Cloning are given below
i) Insertion of isolated gene to the vector
ii) Introduction of recombinant vector to the
host
iii) Isolation of desired
gene
iv) Expression of
recombinant gene in host
v)
Extraction of recombinant gene product
The correct sequence of steps involved are
1. iii, i, iv, ii, v 2. iii, i, ii, iv, v 3. i, ii, iii, iv, v 4. ii, i, iii, iv, v
7. A gene for insulin has
been inserted into a vector for the purpose of obtaining its protein product
only. Such a vector is called
1)
Expression vector 2) suppression vector
3) Storage vector for
genomic library 4) none of the above
8.Expression vectors are
those
1) produce protein
products 2.used for genomic libraries
3) used for chromosome
synthesis 4.used for finger printing
9.E. coli is generally used for gene
cloning because
1) it supports the
replication of recombinant DNA
2) it is easy to transfor
3) it is free from elements
that interferes with replication and
recombination of DNA
4) all of these
10. An ideal plasmid to be
used for recombinant DNA technology must have
1) minimum amount of
DNA 2. relaxed replication control
3) one recognition site for
one restriction endonuclease
4) all of these
11.Transgenic organisms are
1) produced by gene transfer
technology
2)extinct organisms
3) naturally occurring and
endemic
4) produced by traditional
plant breeding technique
12.Transfer
of recombinant plasmid into E. Coli
cells needs
1) heat treatment 2) UV rays treatment
3) Cacl2
treatment 4) lysis
13.Which of the following
statement about a vector is correct
1) all vectors are plasmids
only
2) plasmids, phages can be
used as vectors
3) fungi can also be used as
vectors
4) cyanobacteria can also be used as
vectors
14.Which
of the following statement about plasmids is correct?
1)
plasmids are present in bacteria only
2)
plasmids are present in all organisms
3)
plasmids present in bacteria and phages
4)
plasmids present in plants and animals
15.Which
one of the following statement are not attributed to plasmids
1)
they are circular DNA molecule
2)
they have antibiotic resistant genes
3)
they have the ability of autonomous replication
4)
they have DNA that is as long as chromosomal DNA
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