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14 Jun 2015

CSIR NET Life Sciences Mock Test III

  Edubio       14 Jun 2015


1.Which of the following period corresponds to temperature fall and a cold and dry climate on earth for the first time?
(1) Ordovician period
(2) Silurian period
(3) Carboniferous period
(4) Devonian period


2.In which of the following molecular markers, gel electrophoresis is not required
(1) VNTR
(2) RAPD
(3) SNP
(4) None

3.Match the following products with the microbes which produce them on large scale and find the correct combination
       Product               Microbe
(P) Lysine —            Brevibacterium spp.
(Q) Glutamic acid — Corynebacterium glutamicum
(R) Gibbellic acid — Fusarium mohiliforme
(S) Riboflavin —       Eremothecium ashbyi
(1) Q, R
(2) P, Q
(3) R, S
(4) R, S

4.Pure water does not conduct electricity be cause it
(1 ) Has low boiling water
(2 ) Is almost unionised
(3 ) Is neutral
(4 ) Is readily decomposed

5.A radiolabelled Co2 will release in which of the following case
(1) When acetyl Co-A entering Kreb cycle has radio labelled
(2) Glycine entering glycine decarboxylase system has radiolabelled a C
(3) Glycine entering glycine decorboxylase system has radiolabelled C at the coo-group
(4) When g’ (gamma) c of acetoacetate is radiolabelled during formation of ketone bodies 

6.Choose the mismatch.
(1) Rotenone –– inhibits the transfer of electron the NADH–CoQ reductare
(2) Piericidin A –– Compete with Cyt (a.a3).
(3) Antimycin A –– block electron transport at the level of complex III.
(4) Cyanide –– binds with cytochrome oxidase complex

7.Amino acid residues present in silk fibroin, permitting a close packing of b sheets and an interlocking arrangement of R groups?
(1) Gly, Val
(2) Leu, Arg
(3) Ala, Gly
(4) Gly, His

8. Gene for an enzyme which catalysis synthesis of a secondary metabolite in an angiosperm was transferred to a bacterial expression vector. But it was found that transgene was expressed but a functional enzyme was not synthesized which could be the best possible explanation?
(1) Plant enzyme was not stable in bacteria
(2) Post translational modification did not occur
(3) The promoter used were not appropriate
(4) Both B and C

9. The “Master Regulatory Gene” is located on which chromosomes in humans?                                                                                                                                                       (1) x chromosome
(2) Y chromosome
(3) Both X, Y chromosome
(4) Autosomes

10.  In which of the following combination the name of hormone, its chemical type & its tissue of origin correctly matched?
(1) Aldosterone, Peptide, Pancreas
(2) Glucagon, peptide, cortex Adrenal
(3) ACTH, peptide, adrenal cortex
(4) Vasopressin, Peptide, posterior pituitary

Answers:
1. (4) Temperature fall and a cold and dry climate on earth for the first time corresponds to devonian period.       
 
2. (3) SNP are described as the third generation molecular marker. In RAPD, VNTR gel-based assay are necessary that are time consuming. Single nucleotide polymorphisms (SNPs) represent sites, where DNA sequences differs by a single base. Several non-gel based methods are available for SNP detectio  

3. (3) Correct combination
Glutamic acid –– Corynebacterium glutamicum.
Riboflavin –– Eremothecium ashbyi.  
     
4. (2) Pure water does not conduct electricity because it is almost unionised.

5. (3) A radiolabelled CO2 will release if glycine has radiolabelled C at the – COO¯ group enter into glycine decarboxylase system because during decarboxylation CO2 is released from carboxylic group of glycine.     

6. (2) Piericidin A competes with Q (ubiquinone) and blocks the transfer of electrons at the NADH-CoQ reductase complex        

7. (3) Silk fibrion is rich in Ala and Gly residues, permitting a close packing of b sheets and an interlocking arrangements of R groups     

8. (2) Transegene was expressed but a functional enzyme was not synthesized which means post translational modification did not occur in the products encoded by transcend.     

9. (1) The “master Regulatory gene “ is located on Y chromosome in humans, Sex determining region, Y (SRY) gene is the only gene on Y chromosome that is necessary to initiate testies development.     

10. (4) Vasopressin also known as anti-diuretic hormone is a peptide hormone which is secreted from posterior pituitary                          
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