CSIR NET Life Sciences Mock Test Paper Part-B

2 Mark Questions

1.Each amino acid in a protein is specified by

a.                    several genes

b.                   a promoter 

c.                    an mRNA molecule

d.                   a codon 

2.                 The DNA sequence of a codon in a gene was changed from AAT to AAC. This type

mutation is called       

a.                    Point mutation

b.                   Insertion mutation

c.                    Inversion 

d.                   Deletion 

3.The site where RNA polymerase attaches to the DNA molecule to start the formation of RNA is called a(n) 

a.                    promoter 

b.                   exon

c.                    intron

d.                   GC hairpin 

4.The three nucleotide codon system can be arranged into …………combination 

a.                    16

b.                   20

c.                    64

d.                   128 

5.The TATA box in eukaryotes is a

a.                    core promoter

b.                   -35 sequence

c.                    -10 sequence

d.                   5’ cap

 6.A(n) ………………………… is a piece of DNA with a groups of genes that are transcribed together as a unit

a.                    promoter

b.                   repressor

c.                    operator

d.                   operon 

7.In Sanger-Coulson method the DNA to be sequenced is cloned into

a.                    pBR 322

b.                   pUC 8

c.                    M13 mp8/mp9

d.                   pBR 327 

8.                   If one strand of a DNA molecule has the base sequence ATTGCAT, its complementary

strand will be 

a.                    ATGCAT

b.                   TAACGTA

c.                    GCCATGC

d.                   CGGTACG 

9.                   DNA is made up of building blocks called

a.                    proteins

b.                   bases

c.                    nucleotides

d.                   deoxyribose 

10.               X- ray diffraction experiments conducted by ………………………… led to the

determination of   the structure of DNA.

a.                    Francis Crick

b.                    James Watson

c.                    Erwin Chargaff

d.                   Rosalind Franklin 

11.               Meselson and Stahl proved that 

a.                    DNA is the genetic material

b.                   DNA is made from nucleotides

c.                    DNA replicates in a semiconservative manner

d.                     DNA is a double helix held together with base 

12.               Apoptosis is characterized by

a.                       Programmed cell death 

b.                       Membrane leaky syndrome

c.                       Necrosis

d.                      Cell Cycle Arrest Process 

13.               Yeast Artificial Chromosomes (YACs) are used for cloning

a.                    Large Segment of DNA

b.                   mRNA

c.                    Yeast DNA

d.                   Bacterial DNA

14.                Among the E. coli DNA polymerases, which of the following has a 5'-.3' exonuclease    activity?

a.                     Polyrmerase I

b.                    Polyrmerase II

c.                     Polymerase III

d.                    Polymerase e

15.               Protein structure, the a-helix and b-pleated sheets are examples of

a.                      primary structure

b.                      secondary structure

c.                      tertiary structure

d.                     quaternary structure 

16.               Genetic engineering requires which of the following enzymes?

a.                     B-Galactosidase

b.                     Amylase

c.                     Lipase

d.                    Restriction endonuclease 

17.               Which of the following compounds is used for separation of cells by density gradient methods?

a.                     Caesium sulphate

b.                     Sodium iodide

c.                      Ficoll

d.                     Glycerol 

18.               The selection markers on the plasmid pbr322 confer resistance to

a.                   chloramphenicol and kanamycin

b.                   kanamycin and ampicillin

c.                   kanamycin and tetracycline

d.                  tetracycline and ampicillin 

19.               The dye used in Gram's staining protocol for bacteria is

a.                   eosin

b.                   hematorylin

c.                   iodine and crystal violet

d.                  methylene blue 

20.               Southern blotting detects

a.                   DNA

b.                   RNA

c.                   proteins

d.                  carbohydrates

21.               Transposons (jumping genes) were discovered by

a.                   Temin

b.                   Abelson

c.                   Harvey

d.                  McClintock

22.                The unit of organization and functioning of living systems is

a.                   cell

b.                   nucleus

c.                   plasma membrane

d.                  mitochondria

23.               Endogenous antigens are presented on to the cell surface along with

a.                   MHC-II

b.                   MHC-I

c.                   Fc?receptor

d.                  complement receptor 

24.               In nature, Agrobacterium tumefaciens mediated infection of plant cells leads to                            A.crown gall disease in plants         B. hairy root disease in plants

C.                 transfer of T-DNA into the plant chromosome   D. transfer of Ri-plasmid into the plant cell

a.                   D only 

b.                   A and C only 

c.                   B and D only

d.                   B only 

25.               The nucleotide analogue used in DNA sequencing by chain termination method is

a.                   1',3'-dideoxy nucleoside triphosphate

b.                   2',3'-dideoxy nucleoside triphosphate

c.                   2',4'-dideoxy nucleoside triphosphate

d.                  2',5'-dideoxy nucleoside triphosphate 

26.               Animals store glucose in the form of

a.                   amylose

b.                   glycogen

c.                   glycerol

d.                  guanine 

27.               The respiratory control center of humans is located in the

a.                   blood-brain barrier

b.                   alveoli

c.                   erythrocytes

d.                  brainstem (medulla oblongata) 

28.               The use of antibiotics is a very effective way of destroying pathogenic bacteria (disease causing), but sometimes people do not finish all of their pills. This can result in:

a.                   fewer bacteria

b.                   more bacteria

c.                   bacteria sensitive to the antibiotics

d.                  bacteria resistant to the antibiotics 

29.               Which of the following nitrogenous bases is found in DNA but is not found in RNA?

a.                   adenine

b.                   guanine

c.                   cytosine

d.                  thymine

30.                Which one is called the milk sugar

a.                   Maltose 

b.                   Lactose 

c.                   Sucrose 

d.                  Galactose 

31.               Glycogenesis is the

a.                   Formation of glucose 

b.                   Formation of glycogen

c.                   Breakdown of glucose

d.                  Breakdown of glycogen 

32.               Which element occurs in nucleic acids?

a.                   calcium

b.                   phosphorus

c.                   manganese

d.                  sulfur 

33.               Echocardiogram is a record of

a.                   Ultrasonic measurement in the heart

b.                   Heart’s electrical activity measured on an electrocardiograph

c.                   Ultrasonic measurements in the brain

d.                  Neuronal activity of the brain measured on an electroencephalograph 

34.               Antibodies are secreted by

a.                   T cells 

b.                   B cells 

c.                   Plasma cells 

d.                  None of the above 

35.               Adenosine is a 

a.                   Purine base

b.                   Pyrimidine base

c.                   Nucleotide 

d.                  Nucleoside 

36.               Prions are infectious particles that are comprised entirely of

a.                   DNA

b.                   RNA 

c.                   Proteins 

d.                  Carbohydrates 

37.                If  the  sequence  of  the  DNA  sense  strand  is  5’GATCCTATGCTAC  3’,  then  the transcribed mRNA sequence will be

a.                   5’ GAUCCUAUGCUAC 3’ 

b.                   5’ CUAGGUAUCGAUC 3’

c.                   5’ CAUCGUAUACCUAG 3’

d.                  5’ GUAGCAUAGGAUC 3’

38.                The 20 different amino acids found in proteins are

normally coded by

a.                   59 codons

b.                   60 codons

c.                   61 codons

d.                  63 codons 

39.               How many microliters of 0.1 M solution of sodium chloride will make 10 ml of 5 mM                                             sodium chloride?

a.                   200

b.                   100

c.                   500

d.                  10  

40.               Chromatin loops are formed by periodicattachment of the following onto the nuclear skeleton

a.                   Histones

b.                   MARs

c.                   Promoters

d.                  Introns  

41.               Restriction endonucleases which recognize and cut the same recognition sequence are known as

a.                   Isoschizomers

b.                  Abzymes

c.                   Isozymes

d.                  Isoaccepting Endonucleases 

42.                The most widely used programme for multiple sequence alignment is

a.                   BLAST

b.                  FASTA

c.                   CLUSTAL

d.                  CHIME 

43.               Embryonic stem cells are derived from

a.                   Fertilized Embryo

b.                  Unfertilized embryo

c.                   Ova

d.                  Sperm 

44.               The study of evolutionary relationship is known as

a.                   Eugenics

b.                  Genetics

c.                   Phylogenetics

d.                  Genomics 

45.               Interferon-b is produced by

a.                   Viruses infected sir

b.                  Bacteria infected cells

c.                   Fungi infected cell

d.                  Both bacteria and virus infected cells

46.                Naturally competent bacteria for the transformation of DNA is 

a.                   Bacillus subtilis

b.                  Yersinia pestis

c.                   Mycobacterium tuberculosis

d.                  Escherichia coli 

47.               Hybridoma technology is used to produce 

a.                   Monoclonal Antibodies

b.                  Polyclonal ntibodies

c.                   Monoclonal and polyclonal Antibodies both

d.                  B cells 

48.                Antibody  protein  family  members  have  common  structural  features  are  collectively  known as

a.                   Allergens

b.                  Immunoglobulins

c.                   Antigens

d.                  Haptens 

49.               Fc and Fab fragments of IgG are produced upon digestion with

a.                   chymotrypsin

b.                  papain

c.                   trypsin

d.                  lysozyme 

50.               pH of an aqueous solution is 4. What is its pOH?

a.                   4

b.                  3

c.                   10

d.                  12