Biotechnology- CET Mock Test Papers and Answers

Questions

1. Anaerobic metabolism refers to the generation of ATP:

a) without the involvement of ADP

b) without the use of glycogen

c) without the use of oxygen

d)  in the absence of available oxygen

2. Embedded in the inner membrane of the mitochondrion are:

a) the enzymes of the tricarboxylic acid cycle (Krebs’ cycle)

b) the components of the electron transport chain

c) glycogen molecules

d) triacylglycerol molecules

3. The important function of the fattly acids in cells is

a) construction of cell membranes

b) to be associated in protein synthesis

c) in the breakdown of carbohydrates

d) in energy reactions within the cell

4. How does an animal cell utilize the first law of thermodynamics?

a) By utilisng the heat energy

b) By converting the chemical bond energy in foodstuff to heat energy

c) By creating new form of energy

d) By destroying the existing form of energy

5. Plants and some bacteria utilize solar energy to synthesize organic molecules by

a) photosynthesis

b) kinetic energy

c) potential energy

d) chemical bond energy

6. Glycolysis is the name given to the pathway involving the conversion of:

a) glycogen to glucose-6-phosphate

b) glycogen or glucose to fructose

c) glycogen or glucose to pyruvate or lactate

d) glycogen or glucose to pyruvate or acetyl CoA

7. The structure that facilitates the entry or exit of substances is

a) microtubule

b) plasma membrane

c) nucleus

d) golgi apparatus

8. A cell has an extensive distribution of Golgi apparaus. Therefore, you expect the cell to

a) make a lot of ATP.

b) secrete a lot of material.

c) move actively.

d) perform photosynthesis.

9. Which of the following correctly matches an organelle with its function?

a) nucleus . . . cellular respiration

b) ribosome . . . manufacture of lipids

c) lysosome . . . movement

d) central vacuole . . . storage

10. Dye injected into a plant cell might be able to enter an adjacent cell through a

a) tight junction.

b) microtubule.

c) desmosome.

d) plasmodesma.

11. Innate immune system is present only in

a) vertebrates

b) vertebrates and invertebrates

c) invertebrates

d) none of the above

12. Acquired immunity is characterized by

a) fast response

b) involvement of toll-like receptors

c) memory response

d) non-specificity

13. Complement activation involves

a) both humoral and innate immunity

b) innate immunity alone

c) cell-mediated immunity alone

d) humoral immunity alone

14. Somatic hypermutation and class switching results in

a) diversity in antibody production

b) diversity in cytokine production

c) diversity in complement production

d) diversity in T-cell receptor complex

15. CD4+ T cells require the involvement of

a) MHC III molecule

b) MHC II molecule

c) MHC I molecule

d) Macrophage

16. CD8+ T cells require the involvement of

a) Dendritic cells

b) MHC II molecule

c) MHC I molecule

d) Macrophage

17. The “turnover number” of an enzyme is

a) kcat/ km  

b) k-1

c) kcat          

d) k1/k-1

18. Which type of inhibitor binds to both the free enzyme and the ES complex?

a) Non competitive

b) Uncompetitive

c) Competitive

d) None of the above

19. Find the velocity of an enzyme catalysed reaction, if [S] =Km

a) 1/2 Vmax

b) 1/3 Vmax

c) 2/3 Vmax

d) 1/4 Vmax

20. Which of the following statement about enzymes is TRUE?

a) Catabolic enzymes require energy to degrade large macromolecules.

b) Each enzyme must have a bound cofactor for a reaction to occur.

c) Multienzyme complexes consist of many identical enzyme polypeptides .

d) A holoenzyme consists of an inactive apoenzyme plus a cofactor.

21. α- Amylase hydrolyses

a) glycoprotein

b) α- 1,4 glycosidic bonds

c) peptide bond

d) ester bond

22. Enzyme activity is defined as

a) the amount of enzyme needed to catalyze 1 mole of product

b) the amount of enzyme needed to catalyze 1 micromole of product

c) the amount of enzyme needed to catalyze 1 micromole of product in 1 minute

d) the amount of enzyme needed to catalyze 1 mole of product in 1 minute

23. The purity of an enzyme at various stages of purification is best measured by

a) Total protein  

b) Total enzyme activity

c) Specific activity of the enzyme   

d) Percent recovery of protein

24. Who proposed the existence of enzyme-substrate complex?

a) Charles Woods  

b) Adolph Hayden

c) Charles Adolphe Wurtz   

d) Adolphe Woods

25. What will happen to reaction if activation energy is increased?

a) Rate of reaction decrease

b) Rate of reaction increase

c) Reaction equilibrium shifts forward

d) Reaction equilibrium shifts backward

Answers:

1. c) without the use of oxygen

2. b) the components of the electron transport chain

3. a) construction of cell membranes

4. b) By converting the chemical bond energy in foodstuff to heat energy

5. a) photosynthesis

6. c) glycogen or glucose to pyruvate or lactate

7. b) kinetic energy

8. b) secrete a lot of material.

9. d) central vacuole . . . storage

10. d) plasmodesma

11. b) vertebrates and invertebrates

12. c) memory response

13. a) both humoral and innate immunity

14. a) diversity in antibody production

15. b) MHC II molecule

16. c) MHC I molecule

17. c) kcat         

18. a) Non competitive

19. a) 1/2 Vmax

20. d) A holoenzyme consists of an inactive apoenzyme plus a cofactor.

21. b) α- 1,4 glycosidic bonds

22. c) the amount of enzyme needed to catalyze 1 micromole of product in 1 minute

23. c) Specific activity of the enzyme   

24. c) Charles Adolphe Wurtz  

25. a) Rate of reaction decrease