Gene clonng

• How does the cloning of genes from prokaryotes and from eukaryotes differ?
• What are the properties of a good cloning vector? How are cloning vector cut?
• What is gel electrophoresis, and how is it used in gene cloning?
• What is blunt-end ligation, and what are its advantages? 
• What roles do shotgun cloning and gene banks play in cloning genes from     prokaryotes?
• What are the characteristics of a good recombinant DNA host?
• What are the advantages and disadvantages of using E.coli as a host?